DNA extraction from heavy oil contaminated microcosms
- Usage:edible oil
- Type:Cold & Hot Pressing Machine
- Automatic Grade:Automatic
- Production Capacity:98%-100%
- Voltage:380V/50HZ
- Power(W):22kw
- Dimension(L*W*H):48m*12M*15M(30TPD)
- Weight:10tons
- Certification:CE ISO
- After-sales Service Provided:Engineers available to service machinery overseas
- Product name:vegetable oill Production plant
- Raw material:SS304, carbon steel
- Application:Cooking, Cooking,Cooking...
- Function:making edible oil
- Character:the most professional manufactuer of Cooking oil machine
- Advantage:36 years
- Warranty:365 days
- Color:as you require
- After-sales Service:Service Machine Overseas
- Technology:2016
The suitability of the SW-EL method was validated by extracting DNA from samples as small as 0.5 g from model microcosms comprising soil artificially contaminated with 1 and 10% (w/w) heavy
Effects of Heavy Fuel Oil on the Bacterial Community
- Usage:Cooking
- Type:Cold & Hot Pressing Machine, Screw oil press machine
- Automatic Grade:Automatic
- Production Capacity:450~600kg/h
- Voltage:380V
- Power(W):7.5KW
- Dimension(L*W*H):1.95x1.3x1.9m
- Weight:950kg
- Certification:ISO9001
- After-sales Service Provided:Engineers available to service machinery overseas
- Staff:1-2 people
- Pressure adjustment mode:Worm adjustment
- Material:Stainless steel and Carbon steel
- Plant size:30-50 square meter
after the heavy fuel oil was added to the microcosms, the structure of the active bacterial community was modified, indicating a rapid microbial mat response. Members of the Gammaproteobacteria were initially dominant in the contaminated microcosms. Pseudomonas and Acinetobacter were the main genera representa-tive of this class.
Lucia Lozano | Microbiologist, MSc in microbiology, PhD
- Usage:Cooking oill Mill mill
- Type:Cold & Hot Pressing Machine
- Automatic Grade:Automatic
- Production Capacity:100%
- Voltage:380V
- Power(W):according to capacity
- Dimension(L*W*H):according to capacity
- Weight:changed with the capacity
- Certification:CE and BV
- After-sales Service Provided:Engineers available to service machinery overseas
- Product:Cooking oill Mill mill
- Capacity:1-3000TPD
- Warranty:12 months
- Raw material:oil seed
- Material of equipment:stainless steel and carbon steel
- Manufacturing experience:over 40 years experience in edible oil field
- type:Cooking oill Mill mill
- including:machines,installation,tech consulting after sales
- Automatic:Automatic
- operate:easy and safe
This work describes a new method for extracting genomic DNA from heavy oil-contaminated soils. This method combines soil washes using three washing solutions with enzymatic lysis (SW-EL method).
Methods for microbial DNA extraction from soil for PCR
- Form:Oil
- Part:Seed
- Extraction Type:oil refining machine
- Packaging:Glass Container, Plastic Container
- Grade:grade 1-4
- Raw material:crude Seed oil.
- capacity:10-300TPD oil refining machine
- refining ways:Physical or chemical oil refining machine
- certification:ISO&CE and BV
This paper describes in detail a method for extracting DNA from soil which involves minimal purification prior to PCR amplification . The method is compared to other commonly used DNA extraction methods. A PCR product was obtained rapidly and inexpensively from large amounts of soil, even when contaminated with heavy metals.
Electron acceptor-dependent identification of key
- Usage:Crude Edible oill Production Line
- Type:Cold & Hot Pressing Machine, Crude Edible oill Production Line
- Automatic Grade:Automatic
- Production Capacity:30TPD
- Voltage:380V
- Power(W):Based on Crude Edible oill Production Line capacity
- Dimension(L*W*H):Based on Crude Edible oill Production Line capacity
- Weight:Based on Crude Edible oill Production Line capacity
- Certification:CE,BV,ISO9001
- After-sales Service Provided:Engineers available to service machinery overseas
- Processing capacity:30TPD Crude Edible oill Production Line
- Material:Stainless,Carbonless Steel
- Raw Material:Edible,Edible,etc
- Refining Process:Degumming,Deacidification,Decolorization,Deordorization,Degumming,etc
Previous evidence from a tar-oil-contaminated aquifer site in Germany has shown the establishment of a highly active anaerobic toluene(Invitrogen, Darmstadt, Germany) DNA extract was loaded onto a gradient medium of CsCl (average density 1In the ferric iron SIP microcosms, bulk DNA from 12 C-control gradients was dominated mainly by
Regeneration of textile wastewater deteriorated microbial
Textile dye contamination is a serious concern that reduces soil productivity by destabilizing microbial community structures. Here, we investigated the influence of bioaugmentation on the degradation of a mixture of dyes (MOD) and textile industry effluent (TIE) in soil microcosms using eight different dye-degrading bacteria.
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Selection of functional consortium for crude oil
At the end of the test, the medium samples of each dilution level were mixed and collected for DNA extraction and oil concentration analysis. Total crude oil concentration was measured with the Infrared Spectrophotometry (IR) Method (GB/T16488-1996, China) using infrared photometer oil content analyzer (Model 510, ChinaInvent Instruments, China).
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María Alejandra Bautista - Calgary, Alberta, Canada
DNA Extraction From Heavy Oil Contaminated Microcosms and rpob Gene PCR Amplification Actualidades Biológicas January 1, 2008. This work describes a new method for extracting genomic DNA from
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María Alejandra Bautista - Calgary, Alberta, Canada
DNA Extraction From Heavy Oil Contaminated Microcosms and rpob Gene PCR Amplification Actualidades Biológicas January 1, 2008. This work describes a new method for extracting genomic DNA from
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Frontiers | Molecular and Microbiological Insights on the
DNA Extraction From Original Soils and Enrichments. Total microbial DNA was extracted from original soil samples and from each enrichment step of fungal and bacterial culture/microcosms with the PowerLyzer PowerSoil kit (MoBIO Laboratories, Inc., Carlsbad, CA, United States) according to the manufacturer’s instructions.
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An Improved Method for Soil DNA Extraction to Study the
The need for identification of soil microbial community mainly depends on direct extraction of DNA from soil, a multifaceted environment that is a major pool for microbial genetic diversity. The soil DNA extraction procedures usually suffer from two major problems, namely, inappropriate rupturing of cells and contamination with humic substances.
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Natural gas and temperature structured a microbial
Natural gas and temperature structured a microbial community response to theseveral studies have detected Colwellia in oil-contaminated ice cores or sediments incubated atwas able to respond rapidly, becoming abundant in heavy and light DNA. To become so abundant in the heavy DNA, Colwellia had to consume 13 C-labeled
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Distinct succession patterns of abundant and rare bacteria
were collected for DNA extraction.of pe-troleum and can be used by bacteria as carbon source in these microcosms. Cadmium (Cd) was used because it is a heavy metal that is highly toxic to animals, plants, and microorganisms (Thavamani et al., 2012). The pollutant concentrations used in this experiment are common in oil-contaminated soils
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Identifying and Preventing DNA Contamination in a DNA
CONTAMINATION INTRODUCTION One of the biggest strengths of PCR(e) for DNA typing is the degree to which DNA can be amplified. Starting with a single DNA molecule, millions or billions of DNA molecules can be synthesized after 32 cycles of amplification.
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Isolation and characterization of bacteria from soil
tentatively identified as a Rhodococcus sp., degraded diesel oil contained not only in liquid LB and mineral salts media, but also in paddy soil microcosms supplemented with LB medium. The bioaugmentation capacity of isolate J in soil microcosms contaminated with diesel oil was much higher than that of P. aeruginosa strain WatG. The possibility
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Metagenomic and functional analyses of the consequences
The relationship between microbial biodiversity and soil function is an important issue in ecology, yet most studies have been performed in pristine ecosystems. Here, we assess the role of
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Ph.D. thesis Degradation of monoaromatic hydrocarbons in a
microcosms after 3 and 7 day of incubation. DNA extract was loaded onto a gradient medium of CsCl and centrifuged (180 000 g, ~68 h). Twelve fractions from each gradient were collected from ‘heavy’ to ‘light’. After qualitative and quantitative analysis eight DNA fractions of each gradient were selected for
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Simple DNA extraction protocol for a 16S rDNA study
bial DNA extraction of tropical landfarm soil using only direct lysis of macerated material. Two samples of tropical landfarm soil from a Brazil-ian refinery were analyzed by this protocol (one consisted of crude oil-contaminated soil; the other was continuously enriched for nine months with petroleum).
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Comparison of DNA extraction methods to detect Salmonella
Thus, this study was aimed at identifying the most effective DNA extraction methods suitable for molecular protocols specifically for faecal and pork samples. Therefore different DNA isolation kits were tested to extract Salmonella DNA from artificially contaminated specimens and their performances were evaluated by applying a Real Time PCR kit.
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